Capsule Components of Two Strains of a Granulosis Virus of the Armyworm (Pseudaletia unipuncta): Purification by Affinity Chromatography from Proteinase-free Capsules and Analysis by Peptide Mapping

Abstract

SUMMARY Capsule components of two strains of a granulosis virus (GV) which infect the armyworm (Pseudaletia unipuncta) consist of capsule proteins and, in one strain, a synergistic factor, which had higher affinities for hexylamine than the viral components of the enveloped nucleocapsids. The synergistic factor in the capsule of the Hawaiian GV strain was separated from the capsule components using agarose gel coupled with antibody against the synergistic factor. Analysis by peptide mapping showed that the capsule proteins of the two viruses were similar, but that the synergistic factor differed markedly from them in its constitution.