Abstract
Capsid-like PGA nanoparticles (NPs) allow sustained cell transfection in 2D and 3D configurations.
Highlights
University of Bari, Bari, Italy † Electronic supplementary information (ESI) available
The nanotopographical roughness of our NP surface decreases the repulsive interactions with the cell membrane, improving cell adhesion and uptake. This confirmed that our poly-glycolic acid (PGA) NPs mimic the structure of viral vectors, similar to those used for gene delivery.[32]
We have focused our attention on studies of protein corona formation on PGA NPs, after incubation in fetal bovine serum (FBS) and mouse blood at 37 1C for 1 hour or ON with shaking
Summary
Disadvantages including limits in the size of the gene, vector antigenicity, inflammation and possible insertional mutagenesis.[4,5]. Because of all the characteristics discussed above, PGA represents a promising candidate for the synthesis of new non-viral polymeric nanoparticles for gene delivery applications In this context, we have developed stimuli-responsive PGA NPs for delivery of DNA plasmids encoding EGFP used as a model. The combination of the polyhedron structure of our PGA NPs similar to the capsid of viral vectors and the glass transition temperature of the PGA polymer that at human body temperature gives a liquid-like state producing soft and flexible nanoparticles allows us to increase the efficiency of uptake, thanks to the improved cell adhesion, penetration and distribution in complex 3D structures. To the best of our knowledge, no other studies have reported polymeric NPs with capsid-like structures for which it is possible to combine in one system the sensitivity of poly-cations used to complex the DNA with surface degradation of the outer layers of the NPs
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