Caprine Endometrial Mesenchymal Stromal Stem Cell: Multilineage Potential, Characterization, and Growth Kinetics in Breeding and Anestrous Stages.

Amin Tamadon,Farhad Rahmanifar,Mehdi Dianatpour,Younes Zarezadeh,Davood Mehrabani,Shahrokh Zare

doi:10.1155/2017/5052801

Abstract

The endometrial layer of the uterus contains a population of cells with similar characteristics of mesenchymal stem cells (MSCs). In the present study, caprine endometrial mesenchymal stromal stem cells (En-MSCs) characters and differentiation potential to chondrogenic, osteogenic, and adipogenic cell lines as well as their growth kinetics in breeding and anestrous stages were evaluated. En-MSCs were enzymatically isolated from endometrial layer of the uterus of adult goats and were cultured and subcultured until passage 4. The growth kinetics and population doubling time (PDT) of caprine En-MSCs in breeding and anestrous stages were determined. En-MSCs in passage 4 were used for the karyotyping and differentiation into chondrocytes, osteocytes, and adipocytes. The PDT in anestrus phase was 40.6 h and in cyclic goats was 53 h. En-MSCs were fibroblast-like in all passages. The number of chromosomes was normal (2n = 60) with no chromosomal instability. Chondrogenic, osteogenic, and adipogenic differentiation of En-MSCs was confirmed by staining with Alcian blue, Alizarin red, and Oil Red O, respectively. Caprine En-MSCs demonstrated to be an alternative source of MSCs for cell therapy purposes in regenerative medicine.

Highlights

Friedenstein et al [1] were the first who reported isolation of mesenchymal stem cells (MSCs) from bone marrow
Caprine MSCs have been used in tissue engineering for cartilage regeneration and restoring articular cartilage due to the chondrogenic potential of MSCs [9, 10]
Genetic engineering of caprine MSCs has been done for cell therapy by enhancing differentiation potential of MSCs [11], tracking of transplanted cells using green fluorescent protein [12], and facilitating tissue generation process [13]

Summary

Introduction

Friedenstein et al [1] were the first who reported isolation of mesenchymal stem cells (MSCs) from bone marrow. After that adipose tissue [2], umbilical cord blood [3], and endometrium [4] were other reported tissues as sources of MSCs. MSCs were shown to be undifferentiated clonogenic cells with self-renewal and multilineage differentiation properties [5]. MSCs were shown to be undifferentiated clonogenic cells with self-renewal and multilineage differentiation properties [5] Their uses in regenerative medicine are emerging and can be an alternative choice to promote functional recovery in patients suffering from various diseases that may be the cause of death and permanent disability [6]. Genetic engineering of caprine MSCs has been done for cell therapy by enhancing differentiation potential of MSCs [11], tracking of transplanted cells using green fluorescent protein [12], and facilitating tissue generation process [13]

Methods

Results

Conclusion