Capillary Zone Electrophoretic Separation of Four Novel Compounds Active Against Human Immunodeficiency Virus Type 1

Abstract

The separation of four novel small molecules active in the treatment of acquired immunodeficiency syndrome (AIDS), is presented. The molecules were synthesized by computer-aided design with the property of inhibiting the binding of the human immunodeficiency virus type 1 trans-activator of transcription (HIV-1 Tat) protein to the trans-activation response region (TAR) RNA required for HIV-1 transcription, and thus blocking of the HIV replication cycle. The active compounds were separated to baseline by capillary zone electrophoresis (CZE) under optimized conditions with 0.1 mol L−1 ammonium acetate solution (pH 3.8) as running buffer and UV detection. The method will be used study the interactions between the anti-HIV-1 active molecules and HIV TAR RNA and for rapid screening of these potential drugs for early drug discovery.