Abstract
A method for the analysis of monosaccharide enantiomers of the aldose-type is described. Aminoalditols resulting from derivatization with S-(−)-1-phenylethylamine were subjected to capillary electrophoresis in a borate buffer. Standard on-column UV detection at 200 nm was applied. Studies of the dependence of separation selectivity and electrophoretic mobility on temperature, electrolyte composition and pH were carried out. Single-run resolution of all 16 derivatized aldohexoses and 3 out 4 aldotetroses was achieved at pH 10.3 in a 50 m M borate buffer using acetonitrile as organic modifier.
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