Abstract
Capillary zone electrophoresis (CZE) was developed for quantitative determination of hydroxycitric acid and hydroxycitric acid lactone in herbal products of Garcinia atroviridis Griff. Resolution optimization was investigated by varying type, concentration and pH of buffers. Using the pH 9.2 buffer containing 30 mM Na 2B 4O 7, 90 mM NaH 2PO 4 and 0.5 mM tetradecyltrimethyl ammonium bromide, baseline resolution ( R s > 1.5) was found for all analytes. Advantages of the developed CZE method include simple sample preparation, fast analysis time within 5 min and high accuracy and precision.
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