Abstract
The migration of peripheral blood leukocytes from white leghorn roosters sensitized to mycobacteria was significantly inhibited in the presence of PPD. Positive leukocyte migration inhibition tests (LMIT) preceded positive wattle tests in 11 of 34 chickens. In 1 of 42 chickens a positive wattle test preceded positive LMIT. Repeated injections of PPD alone in some chickens resulted in 20% or more inhibition of migration of leukocytes in the presence of PPD ( P <0.05) even though these chickens had negative dermal reactions. Sera from these chickens contained cytophilic antibodies which rendered normal peripheral blood leukocytes sensitive to inhibition by PPD. Serum from one chicken sensitized to mycobacteria also contained cytophilic antibody. Cytophilic antibody was not found in autologous or homologous sera from control chickens. Rabbit anti-chicken IgG abolished leukocyte migration inhibition due to cytophilic antibodies, but had little or no effect on migration inhibition of sensitized cells. The results of these tests indicate that LMIT in chickens are reproducible and parallel that of the human. The addition of rabbit anti-chicken IgG to the LMIT allowed differentiation of leukocyte migration inhibition due to cell-mediated immunity from that due to cytophilic antibody.
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