Abstract

Protein standards are separated on a reverse-phase liquid chromatography column with 40 μm inner diameter. They are introduced to the gas phase by micro-electrospray and detected by a condensation particle counter. The micro-electrospray needle is a new design which allows for the use of a nanoliter-per-minute makeup flow instead of the conventional microliter-per-minute flow. The observed separation efficiency is very good and the sheath micro-electrospray needle performs flawlessly while the system detection limit is comparable to on-column UV absorption. © 1998 John Wiley & Sons, Inc. J Micro Sep 10: 467–471, 1998

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