Abstract
Flavin adenine dinucleotide and deoxyadenosine monophosphate were measured by capillary micellar electrokinetic chromatography combined with semiconductor laser fluorimetry. Methylene blue was dissolved in a carrier solution as a dye for indirect fluorescence detection. In order to decrease the adsorption of the dye on the capillary surface, a quaternary ammonium salt was added to the solution and its concentration was adjusted above the critical micellar concentration. By this technique, the capillary surface negatively charged by dissociation of a silanol group, was changed into a positive charge with this cationic surfactant. The theoretical plate numbers observed were 2.5 × 10 5−3.0 ×10 5. The detection limit of flavin adenine dinucleotide was 100 fmol, which is several orders of magnitude better than reported values obtained by liquid chromatography based on indirect semiconductor laser fluorescence detection. The dynamic reserve and the transfer ratio were 250 and 4 × 10 −4, respectively. The advantages and the limitations of the method are discussed.
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