Capillary geometry in the soleus muscle of rats cold-acclimatized for 68 generations.

Abstract

The effects of chronic cold exposure on soleus muscle capillarity were examined, particularly in terms of the distribution of arteriolar and venular capillaries and their capillary domain area (CDA) in adult rats exposed to cold for 68 generations (CG; n = 6). These parameters were compared with those obtained from control rats (CON; n = 5) and deacclimatized rats (DCG; n = 4), reared in thermoneutral temperature after being reared for 11 generations in cold. Morphometric data were obtained from muscle cross sections exposed to a double-staining method that stained the arteriolar and venular portions of capillaries blue and red, respectively. In CG, the capillary densities of arteriolar and venular capillaries were significantly greater than that of both CON and DCG (P < 0.05). The CDA of arteriolar, intermediate and venular portions in CG was significantly smaller by 15, 14 and 13%, respectively, than those of respective portions in CON (P < 0.05). Although CDA of arteriolar and venular capillary portions was also smaller in DCG than in CON, the degree of reduction was less in DCG than in CG. The succinate dehydrogenase activity of soleus muscle was significantly greater in CG than in both CON and DCG (P < 0.05). These results suggest that adaptive changes in the oxygen transport system, identified as an increase in the number of arteriolar capillaries and a reduction in the diffusion distance for oxygen, were observed in the soleus muscle after chronic cold exposure. These changes may improve the effective oxygen supply to muscle tissues and enable muscle tissues to promote thermogenesis in the cold atmosphere.