Abstract
AbstractThe triacylglycerol composition of olive oil samples has been determined by stereospecific analysis after partial hydrolysis with ethyl magnesium bromide, derivatization, preparative chiral HPLC, transesterification, and GC quantitation of fatty acid methyl esters. The data obtained for position sn‐2 were compared with those from capillary GC analysis of monoacyl sn‐2‐glycerols after enzymatic lipolysis of triacylglycerols.The determination of triacylglycerols collected by silver ion HPLC and quantified (as fatty acid methyl esters) by GC, together with direct GC analysis on a polar column, have then furnished a comprehensive picture of the triacylglycerol content of olive oil.
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