Abstract
A documented case of “scombroid poisoning” is reported by confirming the presence of high histamine levels in fresh tuna fish tissues by gas chromatography-mass spectrometry. Histamine is confirmed by in situ trimethylsilylation of the tissue extract on a 12-m SP-2100 fused-silica capillary column connected directly to a mass spectrometer ion source operated in the positive ion chemical ionization mode using methane as reactant gas. The trimethylsilyl derivative of histamine is readily resolved by the high-resolution fused-silica capillary column and confirmed in the complex tissue extract by this very simple but specific procedure.
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