Abstract
A method for the direct gas chromatographic analysis without prior hydrolysis has been developed for a series of glycine-conjugated bile acids having oxo or multiple functional groups, including oxo and hydroxyl groups and double bonds. The glycine conjugates without and with the hydroxyl groups were derivatized to their ethyl or methyl esters and ethyl ester-trimethylsilyl or methyl ester-dimethylethylsilyl ethers, respectively, and chromatographed on an aluminum-clad, flexible, fused-silica capillary column coated with a thin film (0.1 μm) of chemically bonded and cross-linked methyl polysiloxane. The change of methylene unit value exerted by derivatization and glycination for each compound is discussed.
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