Capillary electrophoretic separation of weak base enantiomers using the single-isomer heptakis-(2,3-dimethyl-6-sulfato)- β-cyclodextrin as resolving agent and methanol as background electrolyte solvent

Abstract

The sodium salt of the single-isomer, heptakis-(2,3-dimethyl-6-sulfato)- β-cyclodextrin (HDMS- βCD) was used as resolving agent in the capillary electrophoretic (CE) separation of weak base enantiomers in pure methanol background electrolytes (BEs). According to the requirements of the charged resolving agent migration model of CE enantiomer separations (CHARM model), a high buffer-capacity, low pH methanolic BE was created from 25 mM phosphoric acid and 12.5 mM NaOH. In this BE, the solubility of HDMS- βCD was as high as 50 mM, permitting the realization of very high separation selectivities and short separation times for the fully protonated weak base enantiomers.