Abstract
Capillary electrophoresis (CE) and chiral stationary phase (CSP) HPLC methods were investigated for the determination of enantiomeric purity of α 1-adrenoreceptor antagonists related to WB 4101. In the CE study, the enantioseparation of the analytes was performed by studying the effect of different types of cyclodextrin in the buffer, namely heptakis (2,6-di-O-methyl)-β-cyclodextrin (DMCD), hydroxypropyl-β-cyclodextrin (HPCD) and β-cyclodextrin (β-CD). HPCD was found to be the most effective chiral selector in the enantioseparation of all the compounds, with high resolution values. A HPLC method, using immobilised serum protein columns, human serum albumin (HSA) and α 1-acid glycoprotein (AGP), was also investigated. Two benzodioxane racemates were well resolved on a mixed type (50% HSA and 50% AGP) column, with enantioselective binding on AGP column.
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