Capillary electrophoresis for the investigation of two novel aminoalkanol derivatives of 1,7-diethyl-8,9-diphenyl-4-azatricyclo[5.2.1.02,6] dec-8-ene-3,5,10-trione as potential anticancer drugs in water solution and serum.

Abstract

A simple, rapid, capillary zone electrophoresis method was developed and validated for the analysis of two novel aminoalkanol derivatives (I) and (II) of 1,7-diethyl-8,9-diphenyl-4-azatricyclo[5.2.1.02,6 ]dec-8-ene-3,5,10-trione, which were found in earlier studies as potential anticancer drugs. Samples were analyzed to demonstrate the specificity and stability indicating ability of the developed method. The samples were extracted using n-hexane-ethyl acetate mixture in the ratio of 90:10. Electrophoretic separation was performed on a eCAP fused silica capillary (37cm length, 50µm inside diameter) with a 50mM tetraborate buffer as a background electrolyte adjusted to pH=2.5. The separation time of (I) and (II) was achieved within 7min. In addition, analysis of the two compounds in the serum was conducted. Limits of detection of (I) and (II) by UV absorbance at 200nm were achieved in the range of 87.4-92.1ng/mL. The sufficient recovery was observed in the range of 90.3-99.8%. The quantification limits for the compounds (I) and (II) were in the range of 279.71-291.03ng/mL, respectively. The method has been successfully applied to the analysis of compounds (I) and (II) in serum samples.