Capillary electrophoresis fingerprints combined with Linear Quantitative Profiling Method to monitor the quality consistency and predict the antioxidant activity of Alkaloids of Sophora flavescens

Abstract

Alkaloids of Sophora flavescens (ASF) has various pharmacological effects, and it is widely used in clinical practice. The aim of this research was to develop an environmentally friendly methodology that enables not only identification but also the quality consistency assessment of Alkaloids of Sophora flavescens. A background electrolyte composed of 50 mmol/L sodium tetraborate solution, 500 mmol/L boric acid and 1.2 mmol/L citric acid was used to conduct the fingerprint analysis coupled with quantitative determination of three components. Linear quantitative profiling method was used for comprehensive quality discrimination of the test samples from both qualitative and quantitative perspectives, and the 27 batches of samples were well differentiated. In addition, the fingerprint-efficacy relationship between chemical components and antioxidant activity in vitro was established using partial least squares regression model, which provided important medicinal efficacy information for quality control.