Abstract
The feasibility of capillary electrochromatography (CEC) was demonstrated using capillary columns packed with 3-μm silica particles and a mobile phase of organic–aqueous buffer system. The retention mechanisms of basic compounds were explored. Preliminary results indicated that the separation of the basic compounds in the CEC system involved multiple retention mechanisms including reversed-phase, cation-exchange as well as normal-phase mechanisms. In addition, electrophoretic mobility of the solutes also contributed to retention. The effects of experimental parameters such as the volume fraction of the organic modifier, pH value and ionic strength of the buffer on the retention behaviours of the solutes were investigated. A comparison of differences between CEC and CE of separations was also discussed.
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