Abstract
ABSTRACT Employing response surface methodology, partially purified polysaccharides from Sorghum bicolor L. leaf sheath were extracted using synergized ethanol adjunct-treated ionic liquid aqueous two phase system (1-octyl-3-methylimidazolium chloride, [C8mim]Cl and K2CO3) and dual frequency ultrasound-assisted extraction. Under ultrasound conditions of 35 °C, 20&60 KHz, 25 mins an experimental yield of 16% PPS was achieved. Dual frequency ultrasound-assisted dialysis effectively reduced salt content of extracted PPS solution in a liquid membrane. The polysaccharides collected after dialysis maintained primary structures. The introduction of ethanol in the ionic liquid aqueous two phase system therefore ensured an excellent simultaneous extraction and partial purification.
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