Abstract
17s-estradiol (E2) is an endocrine-disrupting chemical (EDC) being a hot debate topic that has been discussed among researchers for a decade due to its unexpected harmful effects on humans and wildlife. In this study, we report facile methods to fabricated label-free aptasensor based on the non-faradic mode by using amine-functionalized graphene oxide polypyrrole (NH2-GO/PPy) modified gold interdigitated electrode (IDE) for direct capacitive detection of 17β-estradiol. The as-prepared NH2-GO/PPy nanocomposites have been successfully characterized by FESEM, AFM, FTIR, and electrochemical impedance spectroscopy (EIS). The results show that NH2-GO/PPy/IDE was able to increase the capacitive current as opposed to bare gold IDE. While E2 binding was detected by a decrease in the electrode capacitance due to aptamer's conformational change towards E2. In addition, the biosensor showed a dynamic range of detection from 100nM to 1 pM with a low detection limit of about 0.13 pM. The recoveries result also presented in this paper were validated using HPLC exhibited high sensitivity for BPA detection and were successfully applied to detect E2 in real samples with good recoveries.
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