Abstract

The use of pupil-plane filters in microscopes has been proposed as a method of producing superresolution. Here it is shown that pupil-plane filters cannot increase the support of the transfer function for a large class of optical systems, implying that resolution cannot be improved solely by adding pupil-plane filters to an instrument. However, pupil filters can improve signal-to-noise performance and modify transfer-function zero crossing positions, as demonstrated through a confocal fluorescence example.

Highlights

  • Point spread function (PSF) engineering using pupil-filters is currently an active area of research [1,2,3,4,5,6,7]

  • Other benefits are possible from pupil filters — a confocal fluorescence example is used to demonstrate benefits in terms of signal-to-noise ratio and imaging with a finite-sized confocal pinhole

  • For a considerable class of optical instruments the use of pupil-plane filters cannot increase the support of the system’s transfer function, which implies that they offer no fundamental increase in achievable resolution despite suggestive spatial domain results

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Summary

Introduction

Point spread function (PSF) engineering using pupil-filters is currently an active area of research [1,2,3,4,5,6,7]. This paper is written to call attention to the fact that the use of pupil filters alone in PSF engineering cannot increase the fundamental achievable resolution for a large class of linear, shift-invariant, far-field optical instruments. A full three-dimensional, vectorial, frequency-domain analysis is used to show the addition of pupil-plane filters will not improve the resolution, as no additional frequency-domain components of the object are passed by the instrument. Other benefits are possible from pupil filters — a confocal fluorescence example is used to demonstrate benefits in terms of signal-to-noise ratio and imaging with a finite-sized confocal pinhole

Transfer function analysis
Performance in the presence of noise
Detector aperture effects and modifying zero-crossing positions
Conclusions
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