CAP (Cbl associated protein) regulates receptor-mediated endocytosis

Abstract

CAP (c-Cbl associated protein)/ponsin belongs to a family of adaptor proteins implicated in cell adhesion and signaling. Here we show that CAP binds to and co-localizes with the essential endocytic factor dynamin. We demonstrate that CAP promotes the formation of dynamin-decorated tubule like structures, which are also coated with actin filaments. Accordingly, we found that the expression of CAP leads to the inhibition of dynamin-mediated endocytosis and increases EGFR stability. Thus, we suggest that CAP may coordinate the function of dynamin with the regulation of the actin cytoskeleton during endocytosis. Structured summary: MINT- 6804322: CAP (uniprotkb: Q9BX66) physically interacts (MI: 0218) with Cbl (uniprotkb: Q8K4S7) and dynamin 2 (uniprotkb: P39052) by pull down (MI: 0096) MINT- 6804285: CAP (uniprotkb: Q9BX66) physically interacts (MI: 0218) with FAK (uniprotkb: O35346), vinculin (uniprotkb: P85972) and dynamin 2 (uniprotkb: P39052) by pull down (MI: 0096) MINT- 6804245, MINT- 6804259, MINT- 6804272: CAP (uniprotkb: Q9BX66) physically interacts (MI: 0218) with dynamin 2 (uniprotkb: P39052) by pull down (MI: 0096) MINT- 6804344: CAP (uniprotkb: Q9BX66) physically interacts (MI: 0218) with dynamin 2 (uniprotkb: P50570) by anti tag coimmunoprecipitation (MI: 0007) MINT- 6804371: dynamin 1 (uniprotkb: P21575) physically interacts (MI: 0218) with CAP (uniprotkb: O35413) by anti bait coimmunoprecipitation (MI: 0006) MINT- 6804446, MINT- 6804464: F-actin (uniprotkb: P60709), CAP (uniprotkb: Q9BX66) and dynamin 2 (uniprotkb: P50570) colocalize (MI: 0403) by fluorescence microscopy (MI: 0416)