Canopy structural changes explain reductions in canopy-level solar induced chlorophyll fluorescence in Prunus yedoensis seedlings under a drought stress condition

Abstract

Drought events have a major impact on vegetation structure and function. Recently, solar-induced chlorophyll fluorescence (SIF) has been widely used to understand the photosynthesis rates of vegetation under drought stress conditions. However, it is still unclear whether the reduction in SIF shown under drought stress conditions is regulated by physiological or structural factors. To understand the underlying reduction mechanism of SIF under drought stress, we conducted an experiment under a drought condition using one-year-old Prunus yedoensis seedlings. We compared the experiment trees with control trees that were not exposed to drought stress. We collected spectral and gas exchange data to monitor physiological changes and scanned the trees with a terrestrial laser scanner to monitor the structural changes. The discrete anisotropic radiative transfer (DART) and Fluspect models were used to simulate canopy-level SIF. We found that drought stress caused leaf-level steady-state fluorescence yield to increase, while maximum photosynthetic rate, stomatal conductance, and the quantum yield of Photosystem II decreased significantly. Regarding the canopy structural changes, the leaf inclination angle distribution of the experiment trees gradually turned toward erectophile over time (55.2 ± 9.3° to 74.7 ± 6.0°; mean ± standard deviation), whereas that of the control trees remained relatively constant (52.9 ± 8.8°). Furthermore, reduction of crown cover of the experiment trees was 3-fold (77.4 ± 9.8%) compared with the control trees (26.0 ± 16.2%). The simulated nadir-view canopy-level SIF of the experiment trees was reduced 2.8-fold compared to the control trees. These findings, obtained specifically from Prunus yedoensis seedlings, indicate that canopy-level SIF reduced due to the canopy structural changes, although leaf-level fluorescence yield increased. Therefore, canopy structural changes should be considered when attempting to understand SIF reduction in drought conditions.