Canopy and understory nitrogen additions did not significantly change the community structure of soil fauna under a mature subtropical forest

Abstract

Increasing nitrogen (N) deposition has seriously harmed the structure and function of ecosystems throughout the world and this problem has been increasing. How N deposition affects soil faunal communities is poorly understood, compared to plant and microbial communities. Canopy and understory N additions of 25 and 50 kg ha−1 year−1 were employed to determine whether the effects of N addition on the soil fauna differ between N released to the canopy or to the understory. Specifically, we examined how the soil fauna survives when N additions produce desynchronized and complex impacts on the soil, microbes and litter quantity under mature subtropical forest ecosystems. Our results showed that no significant differences were observed between the soil faunal communities receiving canopy and understory N additions. This is consistent with our observation that the concentrations of ammonia nitrogen and nitrate nitrogen in the soil did not significantly differ under the two different methods of applying N. There were no observed effects on the litter quantity, soil microbial Phospholipid Fatty Acids or soil physical-chemical properties; therefore, it is not surprising that N treatments for 4 years did not significantly alter the community structure of soil fauna under the mature subtropical forest sites. However, the shifts in seasonal differences in the microbial communities under the N treatments had a positive effect on soil microbial development compared to control, which might also produce a time-delay influence on the relative development of the soil fauna under mature subtropical forest in the future. Further dynamic monitoring is needed to illustrate the possible effects and mechanisms by which increasing N deposition may alter soil faunal development in the future.