Canola oil influence on azoxymethane-induced colon carcinogenesis, hypertriglyceridemia and hyperglycemia in Kunming mice.

Xiao-Qiong He,Jin Zhou,Simon Angelo Cichello,Jia-Li Duan

doi:10.7314/apjcp.2014.15.6.2477

Abstract

Azoxymethane (AOM) is a potent genotoxic carcinogen which specifically induces colon cancer. Hyperlipidemia and diabetes have several influences on colon cancer development, with genetic and environmental exposure aspects. Here, we investigated plasma lipid and glucose concentrations in Kunming mice randomized into four groups; control (no AOM or oil exposure), AOM control, AOM + pork oil, and AOM + canola oil. Aberrant crypt foci (ACF), plasma cholesterol, plasma triglyceride, plasma glucose and organ weight were examined 32 weeks after AOM injection. Results revealed that AOM exposure significantly increased ACF number, plasma triglyceride and glucose level. Further, male mice displayed a much higher plasma triglyceride level than female mice in the AOM control group. Dietary fat significantly inhibited AOM-induced hypertriglyceridemia, and canola oil had stronger inhibitory effect than pork oil. AOM-induced hyperglycemia had no sex-difference and was not significantly modified by dietary fat. However, AOM itself not change plasma cholesterol level. AOM significantly increased liver and spleen weight in male mice, but decreased kidney weight in female mice. On the other hand, mice testis weight decreased when fed canola oil. AOM could induce colorectal carcinogenesis, hypertriglyceridemia and hyperglycemia in Kunming mice at the same time, with subsequent studies required to investigate their genome association.

Highlights

Cancer is a widespread pubic health problem both in developed and developing countries, with environmental pollutants such as 2.5PM, or secondary hand nicotine smoke one of many influences on cancer development affecting government policy (Park et al, 2013), including azoxymethane, which is an indirect mutagen and a specific colonic genotoxic carcinogen (Xiao et al, 1996)
We examined the effects of AOM on colon tumorigenesis, plasma triglyceride level, plasma cholesterol level, and plasma glucose level in Kunming mice to observe whether AOM can induce colon cancer, and change the levels of plasma lipids and plasma glucose in Kunming mice at the same time
The plasma total cholesterol (TC), TG and GLU levels of the four groups were examined at the end of the research after mice were stopped feeding for 12 hours

Summary

Introduction

Cancer is a widespread pubic health problem both in developed and developing countries, with environmental pollutants such as 2.5PM, or secondary hand nicotine smoke one of many influences on cancer development affecting government policy (Park et al, 2013), including azoxymethane, which is an indirect mutagen and a specific colonic genotoxic carcinogen (Xiao et al, 1996). It is a genetic background-dependent colorectal cancer initiator and promoter (Anika et al, 2005). No animal model has been set up so far to induce and study all these diseases in the same animal

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Conclusion