Cannabidiol Application Increases Cutaneous Aquaporin-3 and Exerts a Skin Moisturizing Effect.

Nobutomo Ikarashi,Ryotaro Yoshida,Keito Tabata,Risako Kon,Junzo Kamei,Marina Shiseki,Rina Kimura,Hiroyasu Sakai,Tomofumi Watanabe

doi:10.3390/ph14090879

Nobutomo Ikarashi, Ryotaro Yoshida + Show 7 more

Open Access

https://doi.org/10.3390/ph14090879

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Journal: Pharmaceuticals	Publication Date: Aug 30, 2021
Citations: 16	License type: CC BY 4.0

Affiliation: Ebara (Japan), Hoshi University, Juntendo University

Abstract

Cannabidiol (CBD) is a major nonpsychotropic component of Cannabis sativa with various pharmacological activities. In this study, we investigated the skin moisturizing effect of CBD and its mechanism. A 1% CBD solution was applied daily to skin of HR-1 hairless (Seven-week-old, male) for 14 days. The dermal water content in CBD-treated mice was significantly increased compared to that in the control group. Furthermore, no inflammatory reaction in the skin and no obvious skin disorders were observed. The mRNA expression levels of loricrin, filaggrin, collagen, hyaluronic acid degrading enzyme, hyaluronic acid synthase, ceramide degrading enzyme, and ceramide synthase in the skin were not affected by the application of CBD. However, only aquaporin-3 (AQP3), a member of the aquaporin family, showed significantly higher levels in the CBD-treated group than in the control group at both the mRNA and protein levels. It was revealed that CBD has a moisturizing effect on the skin. In addition, it is possible that increased expression of AQP3, which plays an important role in skin water retention, is a contributor to the mechanism. CBD is expected to be developed in the future as a cosmetic material with a unique mechanism.

Highlights

We investigated ronic acid degrading enzyme, hyaluronic acid synthase, ceramide degrading enzyme, the effect synthase, of CBD onand theAQPs) expression of genes
When a 3% CBD solution was applied to mouse skin, no inflammatory reaction was observed, but Transepidermal Water Loss (TEWL) increased and dermal water content decreased to the same level as in the control group
It is possible that the increased expression of AQP3, which plays an important role in skin water retention, is involved as one of the mechanisms of action

Summary

Introduction

Dermal water content is related to the elasticity of the skin and the formation of wrinkles. Dermal water content is controlled by various moisturizing factors, such as collagen [2], ceramide [3,4], and hyaluronic acid [5,6]. Loricrin [7] and filaggrin [8] are involved in the barrier function of the skin and are important for skin moisturization. In addition to these factors, it has become clear that the water channels known as aquaporins (AQPs) play important roles in skin water retention. Various substances and natural products that increase these moisturizing factors and AQPs have been found and are expected to be used as cosmetic materials

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