Abstract
Canine respiratory coronavirus (CRCoV), identified in 2003, is a member of the Coronaviridae family. The virus is a betacoronavirus and a close relative of human coronavirus OC43 and bovine coronavirus. Here, we examined entry of CRCoV into human rectal tumor cells (HRT-18G cell line) by analyzing co-localization of single virus particles with cellular markers in the presence or absence of chemical inhibitors of pathways potentially involved in virus entry. We also targeted these pathways using siRNA. The results show that the virus hijacks caveolin-dependent endocytosis to enter cells via endocytic internalization.
Highlights
Coronaviruses are enveloped, single-stranded, positivesense RNA viruses belonging to the family Coronaviridae within the order Nidovirales [1]
Human coronaviruses (HCoVs) are associated mainly with relatively mild upper and lower respiratory tract disease; emergence of severe acute respiratory syndrome coronavirus (SARSCoV) in the winter of 2002–2003 in China, and more recently Middle East respiratory syndrome coronavirus (MERS-CoV) in the Middle East, demonstrates the potential threat posed by zoonotic coronaviruses [2,3,4]
Canine respiratory coronavirus (CRCoV) was first identified in 2003 in samples obtained from the respiratory tracts of dogs with canine infectious respiratory disease (CIRD; known as kennel cough) that were housed in animal shelters in the United Kingdom [5]
Summary
Coronaviruses are enveloped, single-stranded, positivesense RNA viruses belonging to the family Coronaviridae within the order Nidovirales [1]. Canine respiratory coronavirus (CRCoV) was first identified in 2003 in samples obtained from the respiratory tracts of dogs with canine infectious respiratory disease (CIRD; known as kennel cough) that were housed in animal shelters in the United Kingdom [5]. CIRD is a contagious disease with high morbidity but CRCoV is closely related to two other betacoronaviruses, bovine coronavirus (BCoV) and HCoV-OC43 (97.3% nucleotide identity in the spike gene for BCoV and 96.9% for OC43 as reported by Erles et al [5]), but is clearly distinct from Canine Enteric Coronavirus (CECoV, previously known as Canine Coronavirus) [5, 7].
Sign-in/Register to view highlights & summary Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
