Abstract
Regenerative medicine has been growing because of the emergent need for tissues/organs for transplants and restorative surgeries. Biological scaffolds are important tools to try to solve this problem. The one used in this reserach was developed by an acellular biological scaffold from canine placenta with a rich source of cellular matrix. After decellularization, the cellular matrix demonstrated structural preservation with the presence of important functional proteins such as collagen, fibronectin, and laminin. We used cells transduced with vascular endothelial growth factor (VEGF) to recellularize this scaffold. It was succeeded by seeding the cells in nonadherent plaques in the presence of the sterelized placenta scaffold. Cells were adhered to the scaffold when analyzed by immunocytochemistry and scanning electron microscopy, both showing sprouting of yolk sac VEGF (YSVEGF) cells. This recellularized scaffold is a promissory biomaterial for repairing injured areas where neovascularization is required.
Highlights
IntroductionThe necessity of tissues and organs for transplants exceeds its availability, taking to search for therapeutic alternatives
The necessity of tissues and organs for transplants exceeds its availability, taking to search for therapeutic alternatives.A new tool as biological scaffolds derived from decellularized tissues and organs, extracellular matrix (ECM) may be useful in human and animal regenerative medicine as an alternative to transplantation
We developed an acellular biological scaffold derived from canine placenta preserving its architecture
Summary
The necessity of tissues and organs for transplants exceeds its availability, taking to search for therapeutic alternatives. A new tool as biological scaffolds derived from decellularized tissues and organs, extracellular matrix (ECM) may be useful in human and animal regenerative medicine as an alternative to transplantation. The ECMs may originate from various tissues such as heart,[2,3] blood vessels,[4,5] skin,[6] tendons,[7] kidney,[8] lung,[9,10] and others In order for this matrix to be considered functional, several molecules should be preserved in its structure, include collagens, glycosaminoglycans, proteoglycans, growth factors, and adhesion proteins such as laminin and fibronectin.[11]. This makes it an important tool for the production of scaffolds for use in regenerative medicine where tissue neovascularization is necessary
Sign-in/Register to view highlights & summary Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
