Abstract
In the present study, virological and serological investigations were performed to determine the presence and prevalence of Canine corona virus (CCoV) infection in dog population in Turkey. Sera samples were analyzed for specific antibodies against CCoV by indirect enzyme linked immunosorbent assay (i-ELISA) while leukocyte samples were inoculated onto monolayers of Madin Darby Canine Kidney permanent cell culture. The cells were examined for viral antigen by direct immunofluorescence (IF) test after third passage. CCoV seropositivity was found in 46 (24.46%) of 188 dogs by indirect ELISA while only one leukocyte sample (0.53%) was detected as antigen positive by IF. Seropositive and antigen identification results were considered as indication of infection. From the results of this study it can be concluded that CCoV infection is widespread in the Turkish dog population and the virus may be attributed to be one of the important viral agents in dogs. In conclusion diagnosis of CCoV is difficult because it can easily be mixed with respiratory, enteric and generalized infections by other viral, bacterial and parasitic agents, but diagnosis and the vaccine application are essential for the control and prevention of CCoV infections.
Highlights
The emergence of human severe acute respiratory syndrome encouraged renewed interest in animal coronaviruses as potential agents of direct and indirect zoonosis and has new attention focused on coronaviruses
Different serologic (Mochizuki et al, 1987; Tuchiya et al, 1991; Elia et al, 2003; Buonavoglia et al, 2006; Le Poder, 2011) and virologic diagnostic methods are widely used to detect the genotypes of Canine corona virus (CCoV) (Costa et al, 2014)
It has been hypothesized that CCoV infection which reported from different region of Turkey may affect dogs in Konya, Isparta and Burdur
Summary
The emergence of human severe acute respiratory syndrome encouraged renewed interest in animal coronaviruses as potential agents of direct and indirect zoonosis and has new attention focused on coronaviruses. Different serologic (indirect ELISA, microneutralization) (Mochizuki et al, 1987; Tuchiya et al, 1991; Elia et al, 2003; Buonavoglia et al, 2006; Le Poder, 2011) and virologic (direct ELISA, cell culture, direct immunofluorescence) diagnostic methods are widely used to detect the genotypes of CCoV (Costa et al, 2014). It has been hypothesized that CCoV infection which reported from different region of Turkey may affect dogs in Konya, Isparta and Burdur. CCoV, first described by Binn et al (1974), can affect both domestic and wild dogs worldwide (Decaro and Buonavoglia, 2008; Priestnall et al, 2014). Mild disease or asymptomatic carriages are probably in many cases common outcomes of
Sign-in/Register to view highlights & summary Sign-in/Register to access full text options 
Free) 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a call
