Candidate Genes Polymorphism and Its Association to Prolificacy in Chinese Goats

Yuanqing He,Cunxia Zhang,Xiaoke Ma,Jun Li,Xiaoyong Liu

doi:10.5539/jas.v2n1p88

Abstract

To find the candidate gene concerned with prolificacy in goats, the most part nucleotide sequence of four genes,including GDF9 gene, BMP15 gene, BMPR1B gene, and INH? gene were identified in three goat breeds for theirprobable association to the high fecundity. The results showed that none of polymorphism of GDF9 gene, BMP15 geneand BMPR1B gene was tested in goats, and one mutation was detected in INH? gene. The genotype distributions ofINH? gene were significantly difference between year-round estrous goat breeds and seasonal estrous goat breedsrevealing the association to the prolificacy in goats.

Highlights

The process of ovarian folliculogenesis is composed of proliferation and differentiation of the constitutive cells in developing follicles [Silva et al, 2005]
The results showed that amplication products had good specificity, which could be directly analyzed by single strand conformation polymorphism (SSCP)
The results showed the primers of BMP15 gene, GDF9 gene and BMPR1B gene had no polymorphism, and the primers of INHα gene showed different genotypes

Summary

Introduction

The process of ovarian folliculogenesis is composed of proliferation and differentiation of the constitutive cells in developing follicles [Silva et al, 2005]. Many years have gone since people have great interest in identification of major genes for prolificacy in ovis aries. A number of natural genetic mutations have been identified with ovulation rates varying between 0 and 4 in sheep breeds including one point mutation in the GDF9 gene (FecGH), five different mutations in the bone morphogenetic protein 15 gene (FecXG, FecXB, FecXI, FecXH, FecXL), and one in the BMPR1B gene (FecB). It was certificated that these mutations had great association to the ovulation rate of different sheep breeds, especially the FecB mutation (Booroola breed) and FecXG (Inverdale breed). These genes were selected again to detect the probably effects on goat prolificacy

Methods

Results

Conclusion