Candidate gene microarray analysis in peripheral blood cells for studying hypertension/obesity.

Abstract

The gene expression of 182 cardiovascular candidate genes was measured in high quality groups of individuals (n = 20) by microarrays to determine whether a subset of genes would discriminate obese and hypertensive individuals, in spite of the existence of a close link between these two cardiovascular risk factors. The results were validated on the 20 subjects used for microarray analysis and on 62 additional individuals by real-time PCR. The first analysis, where patient groups were compared with healthy subjects, revealed 15 out of 182 genes that differed in hypertensive, obese or obesity-related hypertensive individuals. These genes were ALOX5, APOA2, SELL, RGS2, CD14, FPR1, CAMP, DEFA3, DEFA4, CBS, CHRM1, ICAM1, NR1H2, SCNN1B and TGFB1. A second analysis was carried out in which patient groups were compared with each other, demonstrating FPR1 and DEFA3 as being significant genes discriminating patient groups. Furthermore, an analysis stratified by sex revealed that, with the exception of DEFA3, there are no other common genes between men and women. We were able to indentify a number of interesting genes that distinguish patient and healthy subject groups as well as patient groups between them. In addition, it seems that gender plays an important role, at least for some of the genes we tested. These findings may have important implications in the screening and etiology of hypertension or obesity, and could further help to focus on these specific mRNAs as antisense therapy targets.