Abstract
Chlorophyll-deficient mutants have been studied persistently to understand genetic mechanisms controlling metabolic pathways. A spontaneous chlorophyll-deficient lethal mutant was observed in self-pollinated progeny of a soybean cultivar “BSR 101”. Observed segregation patterns indicated single-gene recessive inheritance for this lethal-yellow mutant. The objectives of this investigation were to develop a genetic linkage map of the region containing the lethal-yellow (YL_PR350) gene and identify putative candidate genes for this locus. The YL_PR350 gene was mapped to chromosome 15 and is flanked by BARCSOYSSR_15_1591 and BARCSOYSSR_15_1597. This region physically spans ~153 kb and there are 14 predicted genes that lie in this region. The predicted gene Glyma.15g275900 is an excellent candidate for the YL_PR350 gene as it is homologous to an Arabidopsis gene, At3g08010, which codes for a chloroplast-localized protein (ATAB2) involved in the biogenesis of Photosystem I and II. This thylakoid membrane protein is crucial for photosynthesis in Arabidopsis. Future characterization of the candidate gene may enhance our knowledge about photosynthesis, a complex metabolic process critical for sustainability of plants.
Highlights
Variability is a key component that plays an important role in a greater understanding of genetic frameworks and characterization of genomic regions containing important traits
If the candidate gene operates in a similar fashion to its Arabidopsis homolog, we can conclude that problems with thylakoid membrane development are the cause of this lethal-yellow phenotype
We have identified a chlorophyll-deficient mutant that is completely lethal
Summary
Variability is a key component that plays an important role in a greater understanding of genetic frameworks and characterization of genomic regions containing important traits. Chlorophyll-deficient mutants have been instrumental in identifying target genes, understanding gene regulation and characterizing metabolic pathways [1,2,3,4,5,6,7]. Understanding genetic control of the chlorophyll-deficient mutants can help in identification of different players involved in the photosynthetic mechanism. During evaluation at early stage of development, a spontaneous chlorophyll-deficient lethal mutant was observed in self-pollinated progeny of “BSR 101”. Objective for this investigation were to develop a genetic linkage map of the region containing this lethal-yellow gene and to identify putative candidate genes at this locus
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