Abstract
BackgroundCurrently, no clinically useful biomarkers for radioresistance are available in head and neck squamous cell carcinoma (HNSCC). This study assesses the usefulness of Cell Line Microarray (CMA) method to enhance immunohistochemical screening of potential immunohistochemical biomarkers for radioresistance in HNSCC cell lines.MethodsTwenty-nine HNSCC cell lines were cultured, cell pellets formalin-fixed, paraffin-embedded, and arrayed. Radioresistance features of the cell lines were combined to immunohistochemical stains for p53, NDFIP1, EGFR, stem cell marker Oct4, and PP2A inhibitor CIP2A.ResultsExpression of p53, EGFR or CIP2A did not indicate intrinsic radioresistance in vitro. Stem cell marker Oct4 nuclear positivity and NDFIP1 nuclear positivity was correlated with increased intrinsic radioresistance.ConclusionThe usefulness of CMA in analysis of HNSCC cell lines and discovery of biomarkers is demonstrated. CMA is very well adapted to both testing of antibodies in a large panel of cell lines as well as correlating staining results with other cell line characteristics. In addition, CMA-based antibody screening proved an efficient and relatively simple method to identify potential radioresistance biomarkers in HNSCC.
Highlights
No clinically useful biomarkers for radioresistance are available in head and neck squamous cell carcinoma (HNSCC)
Radiotherapy has a major role in the treatment of head and neck squamous cell carcinoma (HNSCC)
26 UT-SCC cell lines were included, the majority of which were derived from male patients with oral cavity cancer (Table 1)
Summary
No clinically useful biomarkers for radioresistance are available in head and neck squamous cell carcinoma (HNSCC). This study assesses the usefulness of Cell Line Microarray (CMA) method to enhance immunohistochemical screening of potential immunohistochemical biomarkers for radioresistance in HNSCC cell lines. Radiotherapy has a major role in the treatment of head and neck squamous cell carcinoma (HNSCC). Albeit the general radiosensitivity of HNSCC, radioresistance of a subset of the tumors is a major clinical problem requiring further study both for an enhanced mechanistic understanding of radioresistance, and to overcome clinical radioresistance in patient therapy. The inability to correctly identify clinically significant molecular events in cell line studies remains a significant problem, since virtually all in vivo cancer studies are preceded by in vitro cell line investigation into cancer behavior and characteristics. The genomic and molecular diversity of different cell lines complicates the selection of cell lines for in vitro studies
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