Abstract
Morphogenesis of two Canadian Bunyavirus isolates, the snowshoe háre subtype of California encephalitis (CE) virus, and Northway (NOR) virus, has been studied in salivary glands of wild-caught Aedes communis mosquitoes from the Western Canadian Arctic. An Australian flavivirus, Murray Valley encephalitis (MVE) virus has also been studied in these mosquitoes. Female mosquitoes were inoculated intrathoracically with 100-1000 mouse LD50 CE or MVE virus or 300 plaque forming units NOR virus. Mosquitoes were incubated at 13°C for extended periods, after which salivary glands were dissected away from mosquitoe thoraces. With the proboscis attached, glands were fixed with glutaraldehyde and postfixed with osmic acid before embedding in Epon 812. Thin sections of salivary glands were examined, using a Philips EM-300 electron microscope. Infectivity titrations of salivary glands or thoraces of the same batch of mosquitoes were performed whenever possible.
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