Can the regenerative potential of an alkali-free bioactive glass composition be enhanced when mixed with resorbable β-TCP?

Abstract

The addition of resorbable β-tricalcium phosphate (β-TCP) to other bone substitute materials such as hydroxyapatite (HA) has been pointed out as a suitable strategy to enhance the regenerative potential of bone grafts made thereof. To check the generalization of this hypothesis, a new synthetic composite bone graft material consisting of a mixture of 30vol% of pure β-TCP and 70vol% of FastOs®BG (an alkali-free bioactive glass - BG) was prepared and tested in vivo. The in vivo performance of the new synthetic bone graft (30β-TCP-70FastOs®BG) was compared with those of FastOs®BG alone and of adbone®BCP, a biphasic calcium phosphate, consisting of 75% of HA and 25% of β-TCP. Two defects with 4mm diameter were performed in Wistar rats calvaria and filled with the bone graft materials. The animals were sacrificed after 9 weeks of implantation and the calvaria was excised. Empty bone defects were used as negative control. The percentages of new bone formed (von Kossa staining) were always higher in the treated groups (FastOs®BG, 30β-TCP-70FastOs®BG and adbone®BCP) than in empty group. There were differences with statistical significance between empty and FastOs®BG groups and between empty and adbone®BCP groups. But the differences observed between empty and 30β-TCP-70FastOs®BG groups were less remarkable. The results demonstrated the superior bone regeneration ability of FastOs®BG alone, which was not further enhanced by adding β-TCP in the composition, confirming its already proven regenerative potential.