Abstract
BackgroundSome microRNAs are involved in diabetes pathology and some are known to have role in stroke. MiR-503 causes endothelial dysfunction in diabetic patients, predisposing to ischemia. There has been no study evaluating Mir-503 level in diabetic patients with or without ischemic stroke.MethodsWe designed a cross-sectional study to assess and compare serum level of MiR-503 in 4 groups of diabetic patients with ischemic stroke (I), non-diabetic patients with stroke (II), diabetic patients (III), and healthy controls (IV) in acute phase and 3 months later.ResultsOur data analysis showed that mean relative expression of MiR-503 in group (I) was significantly higher than 3 other groups (p < 0.05). The level of miR-503 was related to the patients’ fasting blood glucose, Cholesterol level, NIHSS score and acute–phase modified Rankin Scale (mRS) (r = 0.49, p = 0.001, r = 0.5, p = 0.009, r = 0.45, p = 0.009, r = 0.48, p = 0.003, CI = 95%). Relative expression of miR in patients with mRS ≤ 2 (good outcome) was lower than in patients with mRS > 2 (poor outcome) (p = 0.008). After 3 months, level of miR decreased significantly only in group (I) (p = 0.002). Mean relative expression of miR-503 in chronic phase was not significantly different among groups (p-value> 0.05). There was no relation between miRNA level and mRS in chronic phase.ConclusionHyperglycemia and ischemia together raise the level of MiR-503 acutely but it does not remain at high level after 3 months. Although higher miR was related to more disability in acute phase, it does not affect long-term outcome in ischemic patients. As MiR-503 is stable enough in blood it can be used as a potential diagnostic marker of an ischemic stroke in diabetic patient. Its level also is an indicator of stroke severity and patients’ short-term outcome. It is recommended to study whether antagomiR-503 is a new therapeutic agent reducing the severity of and disability due to stroke.
Highlights
Some microRNAs are involved in diabetes pathology and some are known to have role in stroke
Patients with ischemic stroke who were affected by post-ischemic cerebral hemorrhage or cardiac attack during their hospital stay were excluded from further analysis
Our results showed association between the level of miR-503 and Fasting blood sugar (FBS), Chol, NIHSS score and acute–phase modified Rankin Scale (mRS) (r = 0.49 p-value = 0.001; r = 0.5 p = 0.009; r = 0.45 p = 0.009; r = 0.48 p = 0.003 CI = 95%) but no correlation was seen between the level of miR-503 and FBS, Chol, mRS in acute–phase and NIHSS score of patients in groups (I), (II) and (III) separately
Summary
Some microRNAs are involved in diabetes pathology and some are known to have role in stroke. MiR-503 causes endothelial dysfunction in diabetic patients, predisposing to ischemia. There has been no study evaluating Mir-503 level in diabetic patients with or without ischemic stroke. Hyperglycemia causes oxidative stress, leading to endothelial dysfunction and microvascular complications. The latter is the main cause of ischemic stroke [3]. Diabetic patients with ischemic stroke are MicroRNAs are non-coding, tiny, 20–25 nucleotideslong RNAs; which bind to 3’UTR of the target mRNA leading to mRNA degradation or translation inhibition [7, 8]. The role of microRNAs in progression of diabetes and its complications including micro- and macrovascular dysfunction has been highlighted [9].
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