Abstract
We have recently studied the thermotropic phase behavior of cholesterol-containing binary bilayers of a homologous series of linear saturated phosphatidylcholines (CnPCs) with different hydrocarbon-chain lengths n (n = 14-18) using the Prodan fluorescence spectroscopy and differential scanning calorimetry to construct temperature-composition phase diagrams for those binary bilayers. This review describes our concept on the miscibility between cholesterol and CnPC on the basis of the experimental phase diagrams as well as the distribution of cholesterol within the bilayers derived by applying the so-called superlattice model.
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