Abstract
Dendritic localization of mRNA/RNA involves interaction of cis-elements and trans-factors. Small, non-protein coding dendritic BC1 RNA is thought to regulate translation in dendritic microdomains. Following microinjections into cultured cells, BC1 RNA fused to larger mRNAs appeared to impart transport competence to these chimeras, and its 5′ ID region was proposed as the cis-acting dendritic targeting element. As these ID elements move around rodent genomes and, if transcribed, form a long RNA stem-loop, they might, thereby, lead to new localizations for targeted gene products. To test their targeting ability in vivo we created transgenic mice expressing various ID elements fused to the 3′ UTR of reporter mRNA for Enhanced Green Fluorescent Protein. In vivo, neither ID elements nor the BC1 RNA coding region were capable of transporting EGFP RNA to dendrites, although the 3′ UTR of α-CaMKII mRNA, an established cis-acting element did produce positive results. Other mRNAs containing naturally inserted ID elements are also not found in neuronal dendrites. We conclude that the 5′ ID domain from BC1 RNA is not a sufficient dendritic targeting element for mRNAs in vivo.
Highlights
Dendritic localization of RNAs and localized protein synthesis in neuronal dendritic and axonal processes have received increased attention due to their relevance to synaptic plasticity
There are several hundred mRNAs known to be dendritically localized in neurons [1], including MAP 2a/b [2], the a-subunit of Ca2+/ calmodulin-dependent protein kinase type II (a-CaMKII) [3], the cytoskeleton-associated protein Arc [4,5], protein kinase Mf (PKMf) [6], and some non-protein-coding RNAs such as, ribosomal RNAs [7], earlier seen as polyribosomes [8,9,10,11], BC1, BC200, and G22 RNAs [12,13,14], tRNAs [15], and even miRNAs [16]
ID Elements, including the 59-ID Domain of BC1 RNA, do not Target enhanced green fluorescent protein (EGFP) mRNA to Dendrites in vivo
Summary
Dendritic localization of RNAs and localized protein synthesis in neuronal dendritic and axonal processes have received increased attention due to their relevance to synaptic plasticity. There are several hundred mRNAs known to be dendritically localized in neurons [1], including MAP 2a/b [2], the a-subunit of Ca2+/ calmodulin-dependent protein kinase type II (a-CaMKII) [3], the cytoskeleton-associated protein Arc [4,5], protein kinase Mf (PKMf) [6], and some non-protein-coding RNAs (npcRNAs) such as, ribosomal RNAs [7], earlier seen as polyribosomes [8,9,10,11], BC1, BC200, and G22 RNAs [12,13,14], tRNAs [15], and even miRNAs [16]. Specific trans-acting factors bind cis elements of transported RNAs, enabling them to associate with cytoskeletal filaments and be transported with the aid of motor proteins [22,23]
Sign-in/Register to view highlights & summary Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
