Can heat stress and water deficit affect cotton fiber wax content in field-grown plants?

Abstract

Climate modelling predicts a warmer climate for cotton growing regions in the future. This will impose stresses on cotton plants that may impact on cotton fiber cuticle wax levels, and in turn may make cotton textile products more challenging to scour and dye. While previous research has measured the effect of abiotic stress on cuticle wax in cotton leaves and bracts, no research is known to have been undertaken specifically measuring the impact of abiotic stress on cotton fiber wax content. Five Upland (Gossypium hirsutum L.) cotton genotypes with known different tolerances to abiotic stress, were subjected over two growing seasons, to heat stress and or water deficit during the period when fiber cuticle wax deposition occurred. Total ethanol soluble wax content was determined in mature fiber using a standard protocol. Across all genotypes and treatments, fiber wax content varied between 0.2 and 1.6 %. For Sicot 71, a standard Australian commercial genotype, and for Siokra L23 and CIM-448 two genotypes known to be tolerant to abiotic stress, fiber wax content was either unaffected or decreased following the application of abiotic stress. For CS 50, a genotype with a poor tolerance to water deficit stress, fiber wax content increased following abiotic stress. For Sicala V-2, a genotype with less tolerance to abiotic stress, fiber wax content decreased following either heat stress or water deficit treatments alone, while wax content markedly increased following the combined application of both stress treatments. The genotypic variations observed in fiber wax content and the differences in the direction of the response to stresses suggest that conventional breeding could be used to generate new genotypes with acceptable fiber wax levels adapted to future extreme climates.