Abstract
This study was aimed to compare between the effects of different chemical forms of Zn and cupper[ionic forms (CuSO4), (Zn SO4) and chelated forms of [Cu Zn (II) HEDTA and Cu (II) HEDTA], whereas, HEDTA is N-(hydroxyethyl) ethylenediamine triacetic acid, applied at micromolar concentrations in the nutrient solution] of Phaseolus Vulgaris plants grown hydroponically under conditions of iron deficiency (- Fe) were investigated. Plant variants (– Fe + 2 µM Cu2+) and (– Fe+ 20 µM Zn2+) with extremely strong chlorosis were examined for investigations to take after the recuperation of leaf greening after treatment with Cu(II)HEDTA created leaf greening in the two variations, particularly strong for the recently which created leaf, as it appeared with chlorophyll estimations. Changes of plasma membrane reductase movement (PMRA) in roots after treatment with ionic or chelated copper were followed in (+Fe) and (– Fe) plants. The results show the increment of ferric-chelate reductase action (with substrate of Fe (III) HEDTA). Then, the cupric-chelate Cu (II) HEDTA, connected at similar level in arrangements with (– Fe) plants, kept up the high encouragement of plasma membrane ferric-chelate reductase activity. It can be concluded that the treatment with Cu (II) HEDTA enhanced the development and root plasma membrane reductase activity (PMRA) and additionally iron deficiency reactions of phaseolus plants. Regard to cell compounds increase, measurements of 20 μM of Zn altogether developed the action of the protein superoxide dismutase and peroxidase.
Highlights
Copper and iron are important plant micronutrients with root redox mechanisms and directed their mobilization, uptake, and translocation [2]
The main adaptive process includes a strong increase in plasma membrane (PM) ferric-chelate reductase activity by roots accompanied by enhanced proton release needed for the reduction of Fe (III) to more soluble Fe (II) in the apoplast
It was set up that iron deprivation brought to expanded substance of copper in roots [22], but on the other hand it has been demonstrated that ionic copper created the capacity of ferric-chelate reductase activity (FeChRA) of iron-deficient plants [3, 28, 31]
Summary
Copper and iron are important plant micronutrients with root redox mechanisms and directed their mobilization, uptake, and translocation [2]. The aim of the present study was to examine if copper and zinc in different chemical forms ionic or chelated can ameliorate iron deficient in phaseolus plants? Presence of Cu2+ showed marked increase in POD enzyme been observed in presence of ionic and chelated zinc under Fe sufficient conditions, due to (Fe III reductase) activity as compared with either control or (-Fe) play a role as the receiver of electrons on the plasmid treatment. Fe deficiency caused similar changes in the leaves in the presence of Cu-HEDTA or Zn-HEDTA ferric reductase activities and demonstrated the presence (Table 1) These increases resulted highly significant of redox proteins with similar properties at PM. The deficient plants was related to pH changes in the increment of SOD activity may account for the increased accumulation of superoxide radicals (O2.-) in nutrient solutions during iron starvation and copper treatment. Of the possible explanations for the high inhibition of ferric-reduction by free copper ions in roots under Fe-
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