Abstract
To examine the role of cAMP in the regulation of intracellular free magnesium concentration ([Mg 2+] i), we measured [Mg 2+] i in human erythrocytes by 31P-NMR spectroscopy. (−)-Isoproterenol, forskolin, Bt 2cAMP and 8-bromo-cAMP decreased [Mg 2+] i in human erythrocytes. Bt 2cAMP did not increase the effux rate of Mg 2+ from erythrocytes. HA1004, a potent inhibitor of cAMP-dependent kinases, markedly increased the [Mg 2+] i in a Mg 2+-free buffer solution. Addition of 8-bromo-cGMP or 12- O-tetradecanoylphorbol 13-acetate (TPA) did not affect the [Mg 2+] i. These results suggest that β-adrenergic stimulation and cAMP play an important role in the regulation of [Mg 2+] i in human erythrocytes.
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