Abstract
The present study shows the thorough investigations on optical properties and hydrodynamic diameters of glutathione (GSH) stabilized nanosilver clusters (AgNC) at different stages of synthesis and engineering for the optimized absolute quantum yield to generate fluorescent images of Dalton Lymphoma Ascites (DLA) tumour bearing mice. The initial increment of quantum yield was wavelength dependent and finally it became 0.509 which was due to the camouflaging or entrapment of AgNC in macrophages membranes. The potentiality of macrophages membrane camouflaged silver nanoclusters (AgM) was reflected in the cell viability assay and confocal based live dead cell assay where the AgM has better cell killing effect compared to AgNC with reduced dosage and in vivo mice imaging generated the clear visualization at the tumour sites. Therefore, from the present study, it can be considered that the camouflaged nanosilver can be used for targeted theranostic applications.
Highlights
Silver nanoparticles (AgNPs) are known to have anti-cancer properties, along with a targeting moiety, they can prove to be a good anti-cancer drug by itself[4]
If the slope is considered to represent the average efficiency, the average radiant efficiency for AgNC is camouflaged with macrophage membranes (AgM) at 500 nm excitation would be 1.35 × 107 p/sec/cm2/sr per mg/ml which was higher than the AgNC (1.22 × 107 p/sec/cm2/sr per mg/ml) at same wavelength of excitation
This increment is attributed to the camouflaging of AgNC in liposome like structures that was formed by the macrophage membranes
Summary
Agnishwar Girigoswami , WaficYassine, Palani Sharmiladevi, Viswanathan Haribabu & Koyeli Girigoswami. One of these bio mimicking-inspired targeting methods include using membrane rafts obtained from blood cells with specific interest for the use of macrophage membranes since it contains a receptor known as α4β1 integrin that binds to vascular cell adhesion molecule-1 (VCAM-1)[7,8] These VCAM-1 are over expressed in cancer cells especially of that in renal cell carcinoma (RCC). These macrophage rafts can be used to coat the AgNPs to give it bio-mimicking stealth properties and active targeting capability specific to cancer cells[8] This provides favorable conditions for the use of AgNPs as a therapeutic agent. This study, an attempt was made to understand the potential theranostic ability of AgNCs with macrophage raft (membrane) coating from blood cells
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