Abstract
Diarrhea, occurring due to intestinal flora disturbance, is potentially lethal, and its current treatments have adverse effects such as constipation and vomiting. Camelina sativa oil (CSO) is a cooking ingredient and natural remedy used in several countries; however, its pharmacological effects on intestinal health remain unknown. Here, we explored the CSO treatment effects on intestinal flora in male ICR mice with castor oil-induced diarrhea. The rate and degree of loose stools, the diarrhea index, serum inflammatory indices, fecal short-chain fatty acids (SCFAs), and the diversity and abundance of intestinal flora were measured. Castor oil-administered mice experienced diarrhea, reduced intestinal flora diversity and fecal SCFAs concentrations, altered intestinal flora composition, and increased serum proinflammatory indices. In contrast, CSO treatment relieved diarrhea, improved intestinal flora composition, and increased the relative abundance of Lactobacillus and Lachnospiraceae. Additionally, CSO significantly increased the concentrations of fecal propionic acid, valeric acid, isovaleric acid, and serum sIgA, while it reduced those of serum interleukin-17. These findings suggest that CSO could be a promising preventive agent against diarrhea.
Highlights
Diarrhea results from altered electrolyte absorption/secretion or nonabsorbable/osmotically active substances ingested from foods that accumulate in the intestinal lumen
Md_CSOH groups improved, and the body weights of mice in these groups were slightly higher; the rate of loose stool (0.05 vs. 0.23, p < 0.01; 0.07 vs. 0.23, p < 0.01), degree of loose stool (0.62 vs. 1.68, p < 0.05; 0.62 vs. 1.68 p < 0.01), and diarrhea index (0.07 vs. 0.38, p < 0.01; 0.09 vs. 0.38, p < 0.01) of mice in the Md_MP and Md_CSOH groups were significantly lower than those in the Md group, indicating that Camelina sativa oil (CSO) and MP treatments could effectively inhibit castor oilinduced diarrhea (Figures 2(a) and 2(b))
We demonstrated that preventive CSO treatment markedly ameliorated castor oil-induced diarrhea in mice. e findings regarding CSO-specific mechanisms included the following: (i) improvement of the overall intestinal flora diversity; (ii) modulation of intestinal flora composition, especially elevating the relative abundance of Lactobacillus and Lachnospiraceae; (iii) enhancement of intestinal short-chain fatty acids (SCFAs) concentrations, including propionic acid and isovaleric acid; and (iv) decreased expression of proinflammatory factors along with increased secretory immunoglobulin A (sIgA) expression
Summary
Diarrhea results from altered electrolyte absorption/secretion or nonabsorbable/osmotically active substances ingested from foods that accumulate in the intestinal lumen. It is a potentially lethal disease worldwide, in infants, young children, and immune-compromised patients [1, 2], causing more than 0.5 million deaths of children annually [3, 4]. Diarrhea is a common problem that affects up to 5% of adults [7]; this may lead to dehydration and have negative effects on the quality of life and work productivity [8]. Vomiting and constipation are the common adverse events of racecadotril and loperamide during the treatment of diarrhea; these cannot be effectively alleviated [10]. Antibiotics and antiviral treatments are intended to target specific pathogenic microorganisms; they pose considerable health challenges, which are extensive and unpredictable [11]
Sign-in/Register to view highlights & summary Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callMore From: Evidence-based complementary and alternative medicine : eCAM
Disclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
