Abstract
AbstractAlthough considerable information about calpain has been obtained from studies using isolated enzyme preparations, this does not permit the dynamic assessment of intracellular protease activity during changes in intracellular Ca2+ and pH. Intracellular calpain activity is regulated by complex interactions with the specific inhibitory protein (calpastatin), Ca2+ and phospholipids, conditions which are difficult to duplicate in isolated systems. Thus a method to measure calpain activity over time in living cells would be helpful in studying its regulation and physiological roles.KeywordsProximal TubuleCalpain ActivityDynamic AssessmentWestern Blot ProcedurePage BufferThese keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.
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