Abstract

This overview concerning calorimetric studies of animal cells in vitro firstly highlights some of the thermodynamic principles underlying measurement of heat flux. The resurgence of such measurements is based on refined instrumentation and a growing awareness of the undoubted contribution heat flux measurements can make to the accumulating knowledge of cellular physiology. Direct calorimetry has been employed to investigate medical, pharmacological and lexicological problems, and can be a valuable diagnostic tool. It is advocated, however, that it exercises a greater scientific contribution when it is harnessed to indirect calorimetry, especially measurement of oxygen flux. The calorimetric-respirometric (CR) ratio is explained in terms of its value in thermochemical analysis of cellular physiology based on rigorous thermodynamics.

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