Abstract
BackgroundSilent information regulator 2 related enzyme 1 (SIRT1) is one of the key factors in the mechanism of calorie restriction (CR) extending lifespan of animals. The aim of the study is to investigate if CR prolongs ovarian lifespan in mice through activating SIRT1 signaling.MethodsIn the present study, 21 female C57BL/6 mice were divided into three groups: the control (n = 7), CR (n = 7), and SRT1720 (n = 7) groups. After the 26-week treatment, the number of ovarian follicles at each stage was counted, and Western blot was performed.ResultsThe number of surviving follicles in ovaries of the SRT1720 group was less than that of the CR group but more than that of the normal control (NC) group. The number of atretic follicles in the ovaries of the SRT1720 group was similar to that of the CR group but less than that of the NC group. The number of primordial follicles in the ovaries of the SRT1720 group was less than that of the CR group but more than that of the NC group. The numbers of primary follicles, secondary follicles, antral follicles, and corpora lutea in the SRT1720 group were similar to those in the CR group. Western blot analysis showed that the expression of SIRT1, SIRT6, FOXO3a, and NRF1 proteins was upregulated, and p53 was downregulated in both the CR group and the SRT1720 group compared to the control group.ConclusionsOur results indicate that CR inhibits the activation of primordial follicles and development of follicles at different stages, thus preserving the reserve of follicle pool (at least partly) through activating SIRT1 signaling.
Highlights
Silent information regulator 2 related enzyme 1 (SIRT1) is one of the key factors in the mechanism of calorie restriction (CR) extending lifespan of animals
All mice were alive at the end of the 26-week treatment, and no superficial abnormalities or tumors were found in the abdomen and other parts of the body
After SRT1720 treatment, the energy intake of the SRT1720 group gradually decreased compared to the normal control (NC) group
Summary
Silent information regulator 2 related enzyme 1 (SIRT1) is one of the key factors in the mechanism of calorie restriction (CR) extending lifespan of animals. Mammalian females possess a finite number of primordial follicles at birth, following the formation of the primordial follicle pool. Cohorts of these follicles are recruited to begin maturation but the ova do not proliferate, resulting in recruitment continuing until the primordial follicle population becomes depleted. These processes directly affect the number of oocytes available to a female throughout her reproductive life [1]. CR can prolong female reproductive life [5], maintain the follicle reserve [6], and delay ovarian failure of adult rats [7]. The molecular mechanism of CR involved in ovarian follicle development and ovarian lifespan is not clear
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