Caloric restriction and Spirulina platensis extract against ferrous ion (Fe2+) in the aging of Saccharomyces cerevisiae cells deleted to the SIR2 gene

Fábia Benetti,Telma Elita Bertolin,Jorge Alberto Vieira Costa,Thanise Antunes Dias

doi:10.33448/rsd-v9i8.6210

Abstract

The aging process is aggravated by the presence of a high load of oxidative stress associated with the body's imbalance concerning certain metals, with emphasis on iron. Spirulina platensis extract (SP) and caloric restriction (CR) are nutritional interventions capable to mitigate the effects of aging-related diseases. The objective of this study was to determine the effects of SP and CR against ferrous ion on the aging of Saccharomyces cerevisiae deleted of SIR2 gene. Methods: Saccharomyces cerevisiae standard (WT) and sir2Δ strains, cultured in 2% or 0.5% (CR) glucose YPD media, whether exposed to 0.8 mg/mL SP and 1mM Fe2+. Cell viability and lipoperoxidation were analyzed. Results showed reduced cell survival and increased lipid peroxidation in the SIR2 gene deletion. Statistically significant results were found after aging for WT, SP, CR, SP + Fe2+, CR + Fe2+ treatments. The therapies CR and SP showed a protective effect against ferrous ion.

Highlights

Aging is a complex process that involves genetic, physiological and stochastic factors
The objective of this study was to determine the effects of Spirulina platensis extract (SP) and caloric restriction (CR) against ferrous ion on the aging of Saccharomyces cerevisiae deleted of SIR2 gene
There was a significant decrease in cell survival rate (p

Summary

Introduction

Aging is a complex process that involves genetic, physiological and stochastic factors. In this process, there is an accumulation of damaged lipids, proteins, and DNA that lead to cell or organ dysfunction (Rusu, et al, 2019; da Costa, et al, 2016). Labile iron is one of the compounds that lead to oxidative stress because it catalyzes the formation of highly reactive species (Galaris, et al, 2019). The presence of these metal ions, especially in the brain, can trigger physiological changes that compromise neurons, resulting in neurodegeneration and, the development of diseases such as Alzheimer, Parkinson and Huntington (Wojtunik-Kulesza, 2019)

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