Abstract
Human polymorphonuclear leukocytes in the presence of the Ca 2± ionophore A23187 plus Ca 2+ incorporated exogenously-added [ 3H]acetate or 1-0-[ 3H]-alkyl- sn -glycero-3-phosphocholine (lysoplatelet-activating factor) into platelet-activating factor (PAF,1-0-alkyl-2-acetyl- sn -glycero-3-phosphocholine). The incorporation of these radiolabels into PAF by stimulated neutrophils was inhibited by the calmodulin antagonists, trifluoperazine and N-(6-aminohexyl)-5-chloro-1-naphthalene sulfonamide. These two drugs were active at concentrations similar to their respective binding constants to purified calmodulin, suggesting a possible involvement of calcium-activated calmodulin in PAF biosynthesis.
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