Abstract
Aim. To develop an effective protocol for callus induction from shoot apical meristem in Triticum spelta L. and T. aestivum L. Methods. Plant material: spelt “Europe” and common wheat “Bunchuk”. For this research we used shoot apical meristems from 3-days plants. For callus induction we proposed 4 media with different concentration of 2,4-D, picloram, NAA and AgNO3. Explants were growing in dark during 21 day at + 25 C. Results. Calli were transparent and mild, less than 8 mm. For callus induction positive effect were shown on media with 2,4-D and picloram. At the same time, NAA was not such effective. Conclusions. In our research was shown, that the best media for spelt callus induction should have 2 mg/l 2,4-D and 10 mg/l AgNO3.
 Keywords: callusogenesis, spelt (Triticum spelta L.), common wheat, callus, shoot apical meristem.
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
