Abstract
Mesophyll and hypocotyl protoplasts were isolated from Vigna radiata using a combination of Cellulase Onozuka RIO (1%) and Macerozyme (0·2%). On a modified V-47 medium, 60% of the cultured protoplasts divided and developed into colonies. Protoclones differentiated roots on MS medium supplemented with different auxins and cytokinins; however, shoot differentiation was not obtained. Co-cultivation of protoplasts with wild and shooter mutants of Agrobacterium tumefaciens did not lead to differentiation of plantlets. Lysopine and nopaline dehydrogenase were not detected in any of the selected protoclones.
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